CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF SQUALENE-HOPENE CYCLASE FROM ALICYCLOBACILLUS-ACIDOCALDARIUS

Authors
WENDT KU FEIL C LENHART A PORALLA K SCHULZ GE
Citation
Ku. Wendt et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF SQUALENE-HOPENE CYCLASE FROM ALICYCLOBACILLUS-ACIDOCALDARIUS, Protein science, 6(3), 1997, pp. 722-724
Citations number
11
Categorie Soggetti
Biology
Journal title
Protein science → ACNP
ISSN journal
09618368
Volume
6
Issue
3
Year of publication
1997
Pages
722 - 724
Database
ISI
SICI code
0961-8368(1997)6:3<722:CAPCAO>2.0.ZU;2-X
Abstract
The membrane-associated protein squalene-hopene cyclase from Alicyclob acillus acidocaldarius was overexpressed in Escherichia coli and purif ied by ion exchange and gel permeation chromatography Crystals of thre e interrelated forms were grown by vapor diffusion under identical con ditions. The crystals diffract to about 2.3 Angstrom resolution, but t hey are unstable in the X-ray beam. An interpretable heavy-atom deriva tive was obtained.