BIOCHEMICAL-CHARACTERIZATION OF A VASCULAR SMOOTH-MUSCLE CONTRACTING POLYPEPTIDE PURIFIED FROM PHONEUTRIA-NIGRIVENTER (ARMED SPIDER) VENOM

Authors
MARANGONI S BORGES NCC MARANGONI RA ANTUNES E VIEIRA CA NOVELLO JC DOMONT GB GIGLIO JR OLIVEIRA B DENUCCI G
Citation
S. Marangoni et al., BIOCHEMICAL-CHARACTERIZATION OF A VASCULAR SMOOTH-MUSCLE CONTRACTING POLYPEPTIDE PURIFIED FROM PHONEUTRIA-NIGRIVENTER (ARMED SPIDER) VENOM, Toxicon, 31(4), 1993, pp. 377-384
Citations number
15
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
Toxicon → ACNP
ISSN journal
00410101
Volume
31
Issue
4
Year of publication
1993
Pages
377 - 384
Database
ISI
SICI code
0041-0101(1993)31:4<377:BOAVSC>2.0.ZU;2-B
Abstract
Crude Phoneutria nigriventer venom was fractionated by Sephadex, ion-e xchange and reverse-phase high performance liquid chromatography. One protein (PNV1) with spasmogenic activity in rabbit vascular smooth mus cle was isolated and biochemically characterized. PNV1 has 125 amino a cid residues and a calculated mol. wt of 13,899. Special features of t he amino acid composition of PNV1 are the presence of two disulfide br idges and the high percentage (27%) of Asx and Glx. The N-terminal ami no acid sequence indicates that PNV1 is different from other polypepti des isolated from Phoneutria nigriventer venom.