CLONING AND SEQUENCING OF RAT-LIVER CDNAS ENCODING THE REGULATORY PROTEIN OF GLUCOKINASE

cDNAs encoding the rat liver regulatory protein of glucokinase were cl oned and sequenced. The deduced protein contains 568 amino acids for a molecular mass of 62,867 Da. Northern blot analysis showed the presen ce of a major RNA species of 2.35 kb in rat liver. No signal was obser ved with muscle, brain, heart, testis, intestine or spleen RNA. Recomb inant regulatory protein expressed in Escherichia coli was insoluble a nd inactive, and was presumably contained in inclusion bodies. Western blot analysis showed that the recombinant protein was recognized by a ntibodies raised against regulatory protein purified from rat liver.