MOLECULAR-CLONING AND RESTRICTION ENZYME ANALYSIS OF BOVINE ADENOVIRUS TYPE-3

Bovine adenovirus type 3 (BAV3) is a DNA virus that causes respiratory and gastrointestinal disorders in cattle. The viral genome consists o f a linear double-stranded DNA molecule (35,000 base pairs) with inver ted terminal repeats at each of its 5' molecular ends. We have subclon ed 10 HindIII fragments spanning 4.9-96.0%, 5 EcoRI fragments spanning 3.4-89.5% and 2 XbaI fragments spanning 35.7-82.9% of the BAV3 (strai n WBR-1) genome into the bacterial cloning vector pUC19. The subclonin g of the viral genome facilitated the construction of linear restricti on enzyme maps for BamHI, ClaI, EcoRI, HindIII, KpnI, NotI, NspV, PstI , PvuI, SalI, Xbal and Xhol. In this study we report on the molecular cloning and restriction endonuclease mapping of the BAV3 genome.