HETEROGENEITY OF SPLENIC NATURAL SUPPRESSOR CELLS INDUCED IN MICE BY TREATMENT WITH CYCLOPHOSPHAMIDE

Citation
Jc. Brookskaiser et al., HETEROGENEITY OF SPLENIC NATURAL SUPPRESSOR CELLS INDUCED IN MICE BY TREATMENT WITH CYCLOPHOSPHAMIDE, Immunopharmacology, 25(2), 1993, pp. 117-129
Citations number
32
Categorie Soggetti
Pharmacology & Pharmacy",Immunology
Journal title
ISSN journal
01623109
Volume
25
Issue
2
Year of publication
1993
Pages
117 - 129
Database
ISI
SICI code
0162-3109(1993)25:2<117:HOSNSC>2.0.ZU;2-7
Abstract
Administration of high dose cyclophosphamide (CY, 200 mg/kg body weigh t) to adult mice induces transient, nonspecific suppressor activity in the spleen of treated animals. Characterization of the CY-induced nat ural suppressor (NS) cells which inhibit mixed lymphocyte reactions re vealed a heterogenous population of lymphocytes expressing the CD8 T c ell marker and the B220 B cell marker, as well as cells bearing the gr anulocyte-monocyte marker CD11b. On a cell per cell basis the most pot ent of these suppressors were found to be positive for CD11b. Inhibito ry activity was also detected in the CD8-, CD11b-, B220- compartment o f CY-spleen, suggesting the presence of null NS cells. The fact that s everal phenotypically distinct cell populations contribute to the over all inhibitory effect of CY-spleen cells indicates that natural suppre ssion defines an activity rather than a specific cell type. Interestin gly, NS activity was observed to reside solely within the fraction of CY-spleen that is agglutinable with soybean agglutinin or wheat germ a gglutinin, suggesting that expression of receptors for these plant lec tins is a universal characteristic of CY-induced NS cells, regardless of their lineage. CY-spleen cell-mediated suppression of lymphoprolife rative responses was found to be partially dependent on DNA synthesis and totally dependent on protein synthesis, but did not require cell-c ell contact, indicating the production of soluble suppressor factor(s) .