EFFECT OF ACUTE SALINOMYCIN TIAMULIN TOXICITY ON THE LIPID PEROXIDE AND ANTIOXIDANT STATUS OF BROILER CHICKEN

The combined effect, if any, of salinomycin poisoning and salinomycin- tiamulin interaction on lipid-peroxidative processes and the antioxida tive defence system of the liver was studied in domestic fowl. Male br oilers (28-day-old), reared on a diet containing 60 mg/kg salinomycin, were treated intraoesophageally with salinomycin (140 mg/kg body mass ) or tiamulin (50 mg/kg body mass). Malondialdehyde, reduced glutathio ne and cytochrome P-450 concentrations as well as glutathione peroxida se and catalase activities of the liver were determined. Liver malondi aldehyde concentration rose in the salinomycin-treated group while the amount of cytochrome P-450 increased in both groups treated. Glutathi one concentration and glutathione peroxidase activity of the liver dec reased rapidly but hepatic catalase activity increased in both groups after the treatment. Manifestation of the effect exerted by salinomyci n and salinomycin-tiamulin on lipid-peroxidative processes nearly coin cided with the onset of clinical signs and preceded the increase of he patic cytochrome P-450 concentration. According to the results, the ba ckground of the previously reported incompatibility between salinomyci n and tiamulin is the synergistic effect exerted on the antioxidant (g lutathione) system.