EFFECT OF ANISOMYCIN ON THE DEVELOPMENT OF RAPID TOLERANCE TO ETHANOL-INDUCED MOTOR IMPAIRMENT

Male Wistar rats given a single moderate dose (1.7 g/kg, IP) of ethano l (EtOH), followed by six trials on the moving belt apparatus during t he next hour, showed functional tolerance to the motor-impairing effec ts of a second dose given 24 h later if the first EtOH was preceded an d followed by an injection of saline. The same EtOH dose and intoxicat ed practice did not produce tolerance if the saline injections were re placed by two doses of anisomycin (60 mg/kg each, SC) 15 min before an d 105 min after the first dose of EtOH. This finding suggests that rap id tolerance, like chronic tolerance, requires de novo synthesis of pr otein during a short period immediately related to the test experience .