Changes that occurred in the microstructure of low-moisture, part skim
Mozzarella made with a mixed starter consisting of Streptococcus sali
varius ssp. thermophilus and Lactobacillus delbrueckii ssp. bulgaricus
and coagulated with fermentation-produced chymosin, were examined dur
ing 50 days of ripening at 4-degrees-C. Immediately after manufacture
(day 3), a homogeneous and continuous phase of amorphous paracasein re
presented a three-dimensional protein network in the cheese. A large n
umber of irregularly shaped and sized microcavities were present. Duri
ng 50 days of aging, an increase in the porosity of the defatted parac
asein matrix was apparent. These changes were coincidental with a four
fold increase in water-soluble nitrogen (from approximately 2 to 8 % o
f total N) and hydrolysis of approximately 50 % of alpha(s1)-casein. I
t is suggested that the confluence of adjoining microcavities that occ
urred progressively throughout storage may be due to proteolysis or CO
2 production by the starter culture.