APPLICATION OF INVIVO BIOLUMINESCENCE TO THE STUDY OF IONOPHORETIC ACTION

Ionophores (carbonyl cyanide m-chlorophenylhydrazone; valinomycin; and the hop-derived compounds colupulone, trans-isohumulone and trans-hum ulinic acid) reduced the rate of dodecanal-dependent light emission fr om luxA/B-transformed cells of Lactococcus lactis subsp. diacetylactis F712 when the cells were suspended in a buffered medium (pH 6.4) cont aining glucose. This allowed an assay for ionophores to be devised and permitted measurement of the effects of such compounds on the test or ganism by the use of a non-destructive technique in real time.