DNA CONTENT MEASUREMENTS AND AN IMPROVED IDIOGRAM FOR THE INDIAN MUNTJAC

Citation
Hp. Levy et al., DNA CONTENT MEASUREMENTS AND AN IMPROVED IDIOGRAM FOR THE INDIAN MUNTJAC, Cytometry, 14(4), 1993, pp. 362-368
Citations number
36
Categorie Soggetti
Cytology & Histology","Biochemical Research Methods
Journal title
ISSN journal
01964763
Volume
14
Issue
4
Year of publication
1993
Pages
362 - 368
Database
ISI
SICI code
0196-4763(1993)14:4<362:DCMAAI>2.0.ZU;2-9
Abstract
The Indian muntjac, an asiatic deer, has the lowest diploid chromosome number among mammals (female 2N = 6; male 2N = 7). Using flow cytomet ric quantification of propidium iodide-stained cells, we determined th e DNA content of muntjac cells to be 94% that of human. This suggests that the muntjac may serve as a model for investigation of karyotypic evolution and rearrangement. In order to facilitate future comparative gene mapping studies, computer-aided analysis of digitized metaphase chromosomes allowed development of a detailed Indian muntjac G-banded idiogram incorporating both ISCN-type nomenclature and quantitative es timates of the size of each band and position.