Mc. Roccheri et al., 2-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF STRESS PROTEINS IN PARACENTROTUS-LIVIDUS, Journal of submicroscopic cytology and pathology, 25(2), 1993, pp. 173-179
The synthesis of stress proteins in Paracentrotus lividus embryos has
been analysed by two-dimensional electrophoresis following either heat
shock or ZnSO4 administration. As already shown for the 70-KDa hsp (h
eat shock proteins) a non responsive period, that is before hatching,
exists followed by a responsive period in which up to 16 hsps are synt
hesized after heat shock, 7 of which are also synthesized after ZnSO4
treatment. Some of them pre-existed the stress, but their synthesis in
creases following the stress. Only few of them accumulate in amounts d
etectable by silver staining. The synthesis of an these proteins depen
ds upon RNA synthesis.