2-DIMENSIONAL ELECTROPHORETIC ANALYSIS OF STRESS PROTEINS IN PARACENTROTUS-LIVIDUS

The synthesis of stress proteins in Paracentrotus lividus embryos has been analysed by two-dimensional electrophoresis following either heat shock or ZnSO4 administration. As already shown for the 70-KDa hsp (h eat shock proteins) a non responsive period, that is before hatching, exists followed by a responsive period in which up to 16 hsps are synt hesized after heat shock, 7 of which are also synthesized after ZnSO4 treatment. Some of them pre-existed the stress, but their synthesis in creases following the stress. Only few of them accumulate in amounts d etectable by silver staining. The synthesis of an these proteins depen ds upon RNA synthesis.