PORE-FORMING PROPERTIES OF THE MAJOR 53-KILODALTON SURFACE-ANTIGEN FROM THE OUTER SHEATH OF TREPONEMA-DENTICOLA

Authors
EGLI C LEUNG WK MULLER KH HANCOCK REW MCBRIDE BC
Citation
C. Egli et al., PORE-FORMING PROPERTIES OF THE MAJOR 53-KILODALTON SURFACE-ANTIGEN FROM THE OUTER SHEATH OF TREPONEMA-DENTICOLA, Infection and immunity, 61(5), 1993, pp. 1694-1699
Citations number
23
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
61
Issue
5
Year of publication
1993
Pages
1694 - 1699
Database
ISI
SICI code
0019-9567(1993)61:5<1694:PPOTM5>2.0.ZU;2-G
Abstract
A 53-kDa protein from the outer sheath of the oral spirochete Treponem a denticola was purified to homogeneity and shown to reconstitute chan nels in black lipid bilayer model membranes. The channel had a single- channel conductance of 1.8 nS in 0.1 M KCl, making this the largest po rin channel observed to date (estimated diameter, 3.4 nm). Electron mi crographs of 53-kDa-protein-containing outer sheaths of T. denticola s howed a regular hexagonal array of darker staining pits.