MEMBRANE TRANSLOCATION AND CHANNEL-FORMING ACTIVITIES OF DIPHTHERIA-TOXIN ARE BLOCKED BY REPLACING ISOLEUCINE-364 WITH LYSINE

Authors
CABIAUX V MINDELL J COLLIER RJ
Citation
V. Cabiaux et al., MEMBRANE TRANSLOCATION AND CHANNEL-FORMING ACTIVITIES OF DIPHTHERIA-TOXIN ARE BLOCKED BY REPLACING ISOLEUCINE-364 WITH LYSINE, Infection and immunity, 61(5), 1993, pp. 2200-2202
Citations number
14
Categorie Soggetti
Immunology,"Infectious Diseases
Journal title
Infection and immunity → ACNP
ISSN journal
00199567
Volume
61
Issue
5
Year of publication
1993
Pages
2200 - 2202
Database
ISI
SICI code
0019-9567(1993)61:5<2200:MTACAO>2.0.ZU;2-M
Abstract
A mutant of diphtheria toxin in which Ile-364 was replaced by Lys was at least 500-fold less toxic to Vero cells than the parental toxin. It s ability to undergo low-pH-triggered translocation across the plasma membrane was greatly diminished, as was its ability to form ion-conduc tive channels. In addition, the mutant toxin was inactive in the pH-de pendent killing of Escherichia coli.