L. Monge et al., ROLE OF THE ENDOTHELIUM IN THE RESPONSE TO CHOLINOCEPTOR STIMULATION OF RABBIT EAR AND FEMORAL ARTERIES DURING COOLING, British Journal of Pharmacology, 109(1), 1993, pp. 61-67
The role of the endothelium in the effects of cooling on the response
to cholinoceptor stimulation of the rabbit central ear (cutaneous) and
femoral (non-cutaneous) arteries was studied using 2 mm long cylindri
cal segments. Concentration-response curves for acetylcholine (10(-9)-
10(-5)M), methacholine (10(-9)-10(-5)M) and sodium nitroprusside (10(-
9)-10(-4) M) were isometrically recorded in arteries under conditions,
with and without endothelium or following pretreatment with the nitri
c oxide-synthesis inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME
, 10(-6)-3 x 10(-4) M) at 37-degrees-C and at 24-degrees-C (cooling).
Ear and femoral arteries showed endothelium-dependent relaxation to ac
etylcholine and methacholine at 37-degrees-C and 24-degrees-C. The ext
ent of relaxation of the control ear arteries, but not of the control
femoral arteries, to acetylcholine and methacholine increased during c
ooling. L-NAME (10(-6)-3 x 10(-4) M) reduced in a concentration-depend
ent way the response of ear arteries to acetylcholine at both 37-degre
es-C and 24-degrees-C, this reduction being more potent at 37-degrees-
C. L-Arginine (10(-5)-10(-3) M) reversed in a concentration-dependent
manner the inhibitor effects Of 10(-5) M L-NAME at both temperatures.
Sodium nitroprusside caused a concentration-dependent relaxation in bo
th arteries that was endothelium-independent. However, the extent of r
elaxation to this nitrovasodilator in ear and femoral arteries was low
er at 24-degrees-C. These results suggest that cooling augments the re
activity of cutaneous (ear) arteries, but not that of non-cutaneous (f
emoral) arteries to cholinoceptor stimulation by endothelium-mediated
mechanisms. Cooling could therefore facilitate the stimulated release
of endothelial nitric oxide in cutaneous vessels.