SALT EFFECT ON PARTITION-COEFFICIENT OF GLYCINE, L-VALINE, AND L-PHENYL ALANINE IN N-OCTANOL-WATER SYSTEM

The n-octanol-water partition coefficient is an important property to measure the hydrophobicity of organic compounds, which has been demons trated to be a parameter in studying the conformation of biomolecules in aqueous solutions. The hydrophobic parameters of the amino acid sid e chains of peptides have been evaluated from the n-octanol-water part ition coefficients of amino acids. For biological systems, electrolyte s play an important role on thermodynamic properties. Numerous measure ments of the partition coefficients of amino acids in the n-octanol-wa ter system have been already made but without any salt effect. In this study, we investigated the salt effect on the n-octanol-water partiti on coefficients of glycine, L-valine, and L-phenyl alanine with the us e of a generator column. The electrolytes used were LiCl, NaCl, and Na 2SO4.