FRACTIONATION OF NITROGEN ISOTOPES BY GLUTAMINE-SYNTHETASE ISOLATED FROM SPINACH LEAVES

Authors
YONEYAMA T KAMACHI K YAMAYA T MAE T
Citation
T. Yoneyama et al., FRACTIONATION OF NITROGEN ISOTOPES BY GLUTAMINE-SYNTHETASE ISOLATED FROM SPINACH LEAVES, Plant and Cell Physiology, 34(3), 1993, pp. 489-491
Citations number
15
Categorie Soggetti
Plant Sciences
Journal title
Plant and Cell Physiology → ACNP
ISSN journal
00320781
Volume
34
Issue
3
Year of publication
1993
Pages
489 - 491
Database
ISI
SICI code
0032-0781(1993)34:3<489:FONIBG>2.0.ZU;2-Z
Abstract
The isotopic fractionation of nitrogen in the reaction in vitro of glu tamine synthetase isolated from spinach (Spinacia oleracea L.) leaves was calculated from the changes in natural N-15 abundance (deltaN-15 v alue) of ammonia and amide of glutamine during the incubation. The obs erved isotope fractionation under our conditions was - 16.5 +/- 1.5 pa rts per thousand.