SOLID-PHASE INJECTOR FOR OPEN TUBULAR COLUMN SUPERCRITICAL-FLUID CHROMATOGRAPHY

The design and use of a solid-phase injector for open tubular column s upercritical fluid chromatography (SFC) are reported. For sample intro duction, a liquid sample was loaded on a platinum wire. After the solv ent had evaporated, the wire was inserted into, and sealed in, the inj ector. The chromatographic run was started by introducing the supercri tical mobile phase into the injector. Absolute and relative peak area reproducibilities, as well as retention time reproducibility, of the i njection method were evaluated. The relative standard deviations (% RS Ds) of absolute and relative peak areas were 2.0-4.5 and 0.4-1.8%, res pectively, for both 1- and 2-mul injections. Larger deviations were ob served for compounds that had high vapor pressures. The % RSDs of rete ntion times from six subsequent injections were 0.04-0.06.