MEASUREMENT OF STERYLGLYCOSIDES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH 1-ANTHROYLNITRILE DERIVATIVES

A very sensitive and efficient high performance liquid chromtographic (HPLC) method for measuring 0.5-50 ng of free and acylated sterylglyco sides (FSG and ASG) in oils in the form of their 1-anthroylnitrile der ivatives has been developed. Before to derivatization, the whole stery lglycosides were simply isolated with silica gel cartridge then fracti onated via thin layer chromatography (TLC) into pure FSG and ASG. Deri vatization was done on the FSG molecular species, therefore ASG was co nverted into FSG with mild alkaline hydrolysis. The derivatives separa ted by reversed phase mode were detected at 254 nm. Avenasterylglycosi de can not be separated from isofucosterylglycoside and campesterylgly coside from stigmasterylglycoside. The method was successful with to d ifferent vegetable oils commercially extracted with hexane as well as those laboratory-extracted with a polar solvent mixture.