ISOLATION AND MAPPING OF POLYMORPHIC MICROSATELLITES IN CATTLE

A partial plasmid library with bovine genomic inserts of about 500 bas epairs was screened with a (dC-dA)n.(dG-dT)n oligonucleotide probe for the repeated nucleotide motif (CA)n. Eleven positive clones (0.3% of all colonies screened) were discovered and were subsequently isolated and sequenced. Eight microsatellite loci were analysed, one with eight alleles, one with seven alleles, three with six alleles, one with thr ee alleles and two with two alleles. Six of these microsatellites were mapped by PCR-analysis of a panel of somatic hybrid lines.