THE PROMOTER OF A BRASSICA-NAPUS POLYGALACTURONASE GENE DIRECTS POLLEN EXPRESSION OF BETA-GLUCURONIDASE IN TRANSGENIC BRASSICA PLANTS

Authors
HONG HP GERSTER JL DATLA RSS ALBANI D SCOLES G KELLER W ROBERT LS
Citation
Hp. Hong et al., THE PROMOTER OF A BRASSICA-NAPUS POLYGALACTURONASE GENE DIRECTS POLLEN EXPRESSION OF BETA-GLUCURONIDASE IN TRANSGENIC BRASSICA PLANTS, Plant cell reports, 16(6), 1997, pp. 373-378
Citations number
34
Categorie Soggetti
Plant Sciences
Journal title
Plant cell reports → ACNP
ISSN journal
07217714
Volume
16
Issue
6
Year of publication
1997
Pages
373 - 378
Database
ISI
SICI code
0721-7714(1997)16:6<373:TPOABP>2.0.ZU;2-U
Abstract
A 647-bp 5'-flanking fragment obtained from genomic clone Sta 44G(2) b elonging to a family of polygalacturonase genes expressed in Brassica napus pollen was fused to the beta-glucuronidase (GUS) marker gene. Th is fusion construct was introduced into B. napus plants via Agrobacter ium tukmefaciens transformation. Analysis of the transgenic B. napus p lants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.