SEQUENCE OF THE OPEN READING FRAME OF THE FL01 GENE FROM SACCHAROMYCES-CEREVISIAE

The cloned part of the flocculation gene FLO1 of Saccharomyces cerevis iae (Teunissen, A.W.R.H., van den Berg, J.A. and Steensma, H.Y. (1993) . Physical localization of the flocculation gene FLO1 on chromosome I of Saccharomyces cerevisiae, Yeast, in press) has been sequenced. The sequence contains a large open reading frame of 2685 bp. The amino aci d sequence of the putative protein reveals a serine- and threonine-ric h C-terminus (46%), the presence of repeated sequences and a possible secretion signal at the N-terminus. Although the sequence is not compl ete (we assume the missing fragment consists of repeat units), these d ata strongly suggest that the protein is located in the cell wall, and thus may be directly involved in the flocculation process.