CONTROLLED-RELEASE OF POLYPEPTIDES FROM POLYANHYDRIDES

Authors
RON E TUREK T MATHIOWITZ E CHASIN M HAGEMAN M LANGER R
Citation
E. Ron et al., CONTROLLED-RELEASE OF POLYPEPTIDES FROM POLYANHYDRIDES, Proceedings of the National Academy of Sciences of the United Statesof America, 90(9), 1993, pp. 4176-4180
Citations number
29
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
90
Issue
9
Year of publication
1993
Pages
4176 - 4180
Database
ISI
SICI code
0027-8424(1993)90:9<4176:COPFP>2.0.ZU;2-X
Abstract
The effects of both polymer hydrophobicity and addition of stabilizers on the release and integrity of polymer-encapsulated proteins were st udied. By using very hydrophobic poly[1,3-bis(p-carboxyhydroxy)hexane anhydride] with sucrose as an excipient, both recombinant bovine somat otropin and zinc insulin were released intact over 3 weeks. The releas ed proteins appeared to maintain their integrity as judged by acidic r everse-phase HPLC, size-exclusion HPLC, radioimmunoassay, and conforma tion-sensitive immunoassays. Our results also suggest how polymer hydr ophobicity can be used to enhance protein stability.