A DROSOPHILA G-PROTEIN ALPHA-SUBUNIT, GF-ALPHA, EXPRESSED IN A SPATIALLY AND TEMPORALLY RESTRICTED PATTERN DURING DROSOPHILA DEVELOPMENT

Heterotrimeric guanine nucleotide binding proteins (G proteins) couple receptors for extracellular signals to intracellular second messenger -generating systems. Previous studies have shown that G-protein alpha subunits (Galpha) are expressed in a spatially and temporally restrict ed manner during Drosophila embryogenesis and, thus, may be responsibl e for mediating developmental interactions. We have used the polymeras e chain reaction to search for specific Galpha subunits that function primarily during development and not in the adult fly. Using poly(A)RNA isolated from early pupae (day 1), we have isolated a cDNA coding for a fly Galpha subunit, designated Gfalpha. This subunit is 30-38% i dentical to previously described vertebrate and Drosophila Galpha subu nits and appears to define an additional family of Galpha proteins. Gf alpha transcripts are expressed primarily during embryonic, larval, an d early pupal stages and only at low levels in adult flies. In situ hy bridization studies indicate that Gfalpha transcripts are expressed ma ternally and later during embryogenesis primarily in the developing mi dgut and transiently in the amnioserosa. The Gfalpha gene has been cha racterized and mapped to position 73B of the Drosophila genome.