CONSTRUCTION AND ANALYSIS OF THE LINKING LIBRARY OF CORYNEBACTERIUM-GLUTAMICUM ATCC13032

Citation
Yv. Nikolsky et al., CONSTRUCTION AND ANALYSIS OF THE LINKING LIBRARY OF CORYNEBACTERIUM-GLUTAMICUM ATCC13032, Genetika, 29(4), 1993, pp. 549-555
Citations number
16
Categorie Soggetti
Genetics & Heredity
Journal title
ISSN journal
00166758
Volume
29
Issue
4
Year of publication
1993
Pages
549 - 555
Database
ISI
SICI code
0016-6758(1993)29:4<549:CAAOTL>2.0.ZU;2-O
Abstract
Plasmid vector pGEM3Z was used to construct a linking library of Coryn ebacterium glutamicum ATCC13032. About 180 real linking clones were ob tained. The possibility of making use of the linking clones to compila te NotI-restriction map of C. glutamicum ATCC 13032 genome was demonst rated. Nine pairs of adjusting NotI fragment were revealed when the li nking clones were used as hybridization probes.