COMPARISON OF THE CYTOTOXICITIES OF FUSARIUM METABOLITES AND ALTERNARIA METABOLITE AAL-TOXIN TO CULTURED MAMMALIAN-CELL LINES

Citation
Rf. Vesonder et al., COMPARISON OF THE CYTOTOXICITIES OF FUSARIUM METABOLITES AND ALTERNARIA METABOLITE AAL-TOXIN TO CULTURED MAMMALIAN-CELL LINES, Archives of environmental contamination and toxicology, 24(4), 1993, pp. 473-477
Citations number
13
Categorie Soggetti
Toxicology,"Environmental Sciences
ISSN journal
00904341
Volume
24
Issue
4
Year of publication
1993
Pages
473 - 477
Database
ISI
SICI code
0090-4341(1993)24:4<473:COTCOF>2.0.ZU;2-I
Abstract
Four water-soluble Fusarium metabolites (fumonisin B1, fusaric acid, b utenolide and moniliformin), water-insoluble pigment (8-O-methylbostry coidin), and an Alternaria metabolite (AAL-toxin) were tested for rela tive cytotoxicity to five established mammalian cell lines. Butenolide was the most cytotoxic to all five cell lines. LC50s were; 1 mug/ml t o rat hepatoma (RH) (tumors derived from parenchymal cells), 7 mug/ml to baby hamster kidney (BHK-21) fibroblast cells, and 15 mug/ml to McC oy mouse (MM) fibroblast cells: LC100s were 1 mug/ml to Chinese hamste r ovary (CHO) fibroblast cells, and 5 mug/ml to dog kidney (MDCK) fibr oblast cells. Fusaric acid was cytotoxic to the MDCK, MM, RH, and CHO cell lines; moniliformin was cytotoxic to the RH, CHO, and MDCK, cell lines. The pigment, however, was cytotoxic only to RH and CHO cell lin es. Fumonisin B, and a related toxin, AAL-toxin, at a high dose level (100 mug/ml) were not cytotoxic to the RH, BHK, MM, CHO and MDCK cell lines. T-2 toxin was used as a positive control, and inhibited all cel l lines at the nanogram level. The difference in response of these fiv e cell lines to the toxic metabolites, that were noted in this study, was then used to evaluate nine HPLC fractions obtained from a methanol -water extract of an F. moniliforme culture. The results indicated tha t this type of cytotoxicity assay may be useful in following the isola tion of metabolites from extracts of Fusarium culture, especially F. m oniliforme.