REGIONAL MAPPING OF THE ROWETT NUDE GENE (RONU) TO RAT CHROMOSOME 10Q24-]Q32 BY LOCALIZING LINKED SYB2 AND GH LOCI

The Rowett nude gene (RONU) has been mapped on rat chromosome (Chr) 10 by linkage analysis using (ACI x F344/N-RONU/RONU)F1 x F344/N-RONU/RO NU backcross progeny. The gene order on the chromosome was RR92 - (16. 1 cM) - RR24 - (17.9 cM) - MYHSE (myosin heavy chain, embryonic) - (1. 0 cM) - SYB2 (synaptoBrevin 2) - (1.0 cM) - SHBG (sex hormone-Binding globulin) - (4.0 cM) - RONU (Rowett nude) - (29.0 cM) - AEP (anion exc hange protein), PPY (pancreatic polypeptide) - (3.0 cM) - ACE (angIote nsin I converting enzyme), GH (growth hormone). The RONU locus was loc alized to 10q24-->q32 by fluorescence in situ hybridization of the clo sely linked SYB2 and loosely linked GH loci on the opposite side. Cons erved linkage of homologous loci mapped to rat Chr 10 and mouse Chr 11 supports the hypothesis that the RONU locus is a rat homolog of the m ouse nu locus.