THE FUNCTIONAL-PROPERTIES OF HEMOGLOBIN MODIFIED WITH MELLITIC DIANHYDRIDE - POSSIBLE APPLICATIONS AS A BLOOD SUBSTITUTE

Authors
CURRELL DL CHOW R YIMENU T
Citation
Dl. Currell et al., THE FUNCTIONAL-PROPERTIES OF HEMOGLOBIN MODIFIED WITH MELLITIC DIANHYDRIDE - POSSIBLE APPLICATIONS AS A BLOOD SUBSTITUTE, Biomaterials, artificial cells, and immobilization biotechnology, 21(2), 1993, pp. 153-162
Citations number
9
Categorie Soggetti
Engineering, Biomedical","Material Science
Journal title
Biomaterials, artificial cells, and immobilization biotechnology → ACNP
ISSN journal
10557172
Volume
21
Issue
2
Year of publication
1993
Pages
153 - 162
Database
ISI
SICI code
1055-7172(1993)21:2<153:TFOHMW>2.0.ZU;2-S
Abstract
Human oxyhemoglobin reacts with mellitic dianhydride to produce a modi fied protein which shows a reduced oxygen affinity over a wide pH rang e, a reduced but significant cooperativity, a reduced Bohr effect and no response to the allosteric effectors: chloride, clofibric acid or i nositol hexaphosphate. The amount of crosslinking in the modified hemo globin is approximately 22% suggesting promise as a blood substitute. Reaction of deoxyhemoglobin with mellitic dianhydride produces a modif ied protein with reduced response to clofibric acid and a decrease in oxygen affinity in the presence of inositol hexaphosphate.