NONSPECIFIC ADSORPTION AND COVALENT COUPLING OF HEPARIN ON POLYACRYLATE BASED MICROBEADS

Polyacrylate based microbeads were prepared by copolymerization of fou r different acrylate monomers, namely 2-hydroxyethylmethacrylate (HEMA ), ethyleneglycoldimethacrylate (EGDMA), methylmethacrylate (MMA) and dimethylaminoethylmethacrylate (DMAEMA).These beads were further activ ated with CNBr at alkaline pH. The extend of nonspecific adsorption an d covalent coupling of heparin on these beads were investigated in a b atch reactors at different temperatures. The effects of initial concen trations of activation agent and heparin were also studied. Nonspecifi c heparin adsorption on the microbeads containing DMAEMA was significa ntly higher than the others. Nonspecific adsorption decreased with inc reasing temperature. Heparin was covalently coupled on CNBr activated microbeads. The amount of coupled heparin increased by increasing conc entration of CNBr.