GENOMIC MISMATCH SCANNING - A NEW APPROACH TO GENETIC-LINKAGE MAPPING

Genomic mismatch scanning (GMS) is a new method of genetic linkage ana lysis that does not require conventional polymorphic markers or gel el ectrophoresis. GMS is ideally suited to affected-relative-pair mapping . DNA fragments from all regions of identity-by-descent between two re latives are isolated based on their ability to form extensive mismatch -free hybrid molecules. The genomic origin of this selected pool of DN A fragments is then mapped in a single hybridization step. Here we dem onstrate the practicality of GMS in a model organism, Saccharomyces ce revisiae. GMS is likely to be applicable to other organisms, including humans, and may be of particular value in mapping complex genetic tra its.