INTERLEUKIN 1-INDUCED CANCER CELL ENDOTHELIAL-CELL ADHESION INVITRO AND ITS RELATIONSHIP TO METASTASIS INVIVO - ROLE OF VESSEL WALL 13-HODESYNTHESIS AND INTEGRIN EXPRESSION

Previously, we have demonstrated that stimulation of endothelial cells (ECs) with interleukin-1alpha (IL-1alpha) enhances the synthesis and expression of the vitronectin receptor (VnR), promotes VnR-dependent a dhesion of human A549 adenocarcinoma cells to ECs, and is associated w ith decreased EC 13-hydroxyoctadecadienoic acid (13-HODE) synthesis in vitro. To determine whether these observations are relevant in vivo, we examined the acute retention and subsequent metastasis of intraveno usly-injected B16F10 melanoma cells in murine lungs, in relation to ve ssel wall 13-HODE. In C57BL/6 mice pretreated with IL-1alpha, vessel w all 13-HODE was decreased and B16F10 lung entrapment and metastasis we re increased. The latter two events were blocked by pretreating the an imals with the GRGDS peptide. These data suggest a relationship betwee n vessel wall 13-HODE synthesis, adhesion molecule expression, and adh esion of B16F10 cells to the endothelium.