FRUCTO-OLIGOSACCHARIDES FROM URGINEA-MARITIMA

Fructo-oligosaccharides from red squill (Urginea maritima) were isolat ed by precipitation with methanol, GPC on Biogel P2/P4, and reversed-p hase HPLC. Structures of the tri- and tetra-saccharides were verified by the reductive cleavage method. A tetrasaccharide that contained bot h (2 --> 1)- and (2 --> 6)-linked beta-D-Fruf residues was isolated. T he higher fractions from GPC were analysed by the reductive cleavage m ethod without prior purification by reversed-phase HPLC. The mode of b iosynthesis of sinistrin is discussed.