SIMULTANEOUS MULTIANALYTE NUCLEIC-ACID DETECTION FOR GASTROINTESTINALBACTERIAL PATHOGENS USING GENESTAR TECHNOLOGY

Authors
WU LX COOMBS J MALMSTROM S GLASS M
Citation
Lx. Wu et al., SIMULTANEOUS MULTIANALYTE NUCLEIC-ACID DETECTION FOR GASTROINTESTINALBACTERIAL PATHOGENS USING GENESTAR TECHNOLOGY, Clinics in laboratory medicine, 17(1), 1997, pp. 129
Citations number
32
Categorie Soggetti
Medical Laboratory Technology
Journal title
Clinics in laboratory medicine → ACNP
ISSN journal
02722712
Volume
17
Issue
1
Year of publication
1997
Database
ISI
SICI code
0272-2712(1997)17:1<129:SMNDFG>2.0.ZU;2-K
Abstract
The use of Gene-based simultaneous target amplification and recognitio n (GeneSTAR) technologies allows for the rapid detection of five diffe rent bacterial gastrointestinal pathogens from stool sample. The proce ss involves DNA isolation, multianalyte polymerase chain reaction (PCR ) amplification and single base mismatch detection in a microtiter pla te format. The analysis is instrument compatible and can be completed in less than five hours.