A. Fourquet et al., EFFECTS OF LOW-DOSE TOTAL-BODY IRRADIATION (LDTBI) AND RECOMBINANT HUMAN INTERLEUKIN-2 IN MICE, Radiotherapy and oncology, 26(3), 1993, pp. 219-225
Citations number
14
Categorie Soggetti
Oncology,"Radiology,Nuclear Medicine & Medical Imaging
10-16-Week-old female BALB/c mice received low dose total body irradia
tion (LDTBI) in one fraction immediately before the beginning of treat
ment with recombinant human interleukin-2 (rlL-2). LDTBI prevented in
a dose-dependent manner the weight increase of the spleen, liver and l
ungs induced by fluid extravasation provoked by rIL-2 injections. It a
lso limited the increase of the number of mononuclear cells in the spl
een induced after in vivo treatment with rIL-2. Immunofluorescence ana
lysis of spleen cells revealed that LDTBI decreased the relative sIgM cell number in spleen, while the relative numbers of Lyt-1+, Thy-1+ a
nd L3T4+ cells were increased, indicating that a T and/or NK populatio
n, radioresistant to LDTBI, could still proliferate under rIL-2 stimul
ation in vivo. Such lymphocytes were capable of in vitro lysis of YAC
cells in a 4-hour Cr-51 release assay, as well as lymphokine-activated
killer (LAK) cells obtained in mice treated with rIL-2 alone. Thus, L
DTBI given prior to rIL-2, yet preserving the cytotoxic capacity of th
e LAK cells activated by rIL-2, could prevent the vascular leak syndro
me toxicity induced by rIL-2 injection.