DISTANCE-DEPENDENT FLUORESCENCE QUENCHING OBSERVED BY FREQUENCY-DOMAIN FLUOROMETRY

Authors
LAKOWICZ JR KUSBA J SZMACINSKI H JOHNSON ML GRYCZYNSKI I
Citation
Jr. Lakowicz et al., DISTANCE-DEPENDENT FLUORESCENCE QUENCHING OBSERVED BY FREQUENCY-DOMAIN FLUOROMETRY, Chemical physics letters, 206(5-6), 1993, pp. 455-463
Citations number
29
Categorie Soggetti
Physics, Atomic, Molecular & Chemical
Journal title
Chemical physics letters → ACNP
ISSN journal
00092614
Volume
206
Issue
5-6
Year of publication
1993
Pages
455 - 463
Database
ISI
SICI code
0009-2614(1993)206:5-6<455:DFQOBF>2.0.ZU;2-R
Abstract
We describe frequency-domain measurements of the time-dependent intens ity decays of p-bis[2-(5-phenyloxazolyl)] benzene (POPOP) when collisi onally quenched by carbon tetrabromide (CBr4). The frequency-domain da ta reveal the existence of a quenching rate constant which depends exp onentially on the fluorophore-quencher separation. A distance-dependen t quenching rate explains the decadesold observation of upward curvatu re in Stem-Volmer plots.