MICROTUBULE-MEDIATED TRANSPORT OF INCOMING HERPES-SIMPLEX VIRUS-1 CAPSIDS TO THE NUCLEUS

Citation
B. Sodeik et al., MICROTUBULE-MEDIATED TRANSPORT OF INCOMING HERPES-SIMPLEX VIRUS-1 CAPSIDS TO THE NUCLEUS, The Journal of cell biology, 136(5), 1997, pp. 1007-1021
Citations number
67
Categorie Soggetti
Cell Biology
Journal title
ISSN journal
00219525
Volume
136
Issue
5
Year of publication
1997
Pages
1007 - 1021
Database
ISI
SICI code
0021-9525(1997)136:5<1007:MTOIHV>2.0.ZU;2-G
Abstract
Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported acros s the cytosol to the nuclear pore complexes, where the viral DNA genom es are released into the nucleoplasm. Using biochemical assays, immuno fluorescence, and immunoelectron microscopy in the presence and absenc e of microtubule depolymerizing agents, it was shown that the cytosoli c capsid transport in Vero cells was mediated by microtubules. Antibod y labeling revealed the attachment of dynein, a minus end-directed, mi crotubule-dependent motor, to the viral capsids. We propose that the i ncoming capsids bind to microtubules and use dynein to propel them fro m the cell periphery to the nucleus.