El. Kruger et Cd. Pappas, GEOGRAPHIC-VARIATION OF CUTICULAR HYDROCARBONS AMONG 14 POPULATIONS OF AEDES-ALBOPICTUS (DIPTERA, CULICIDAE), Journal of medical entomology, 30(3), 1993, pp. 544-548
Cuticular hydrocarbons were used to differentiate among fourteen Aedes
albopictus (Skuse) populations from Asia, Brazil, and the United Stat
es. Forty cuticular hydrocarbon peaks from each North American populat
ion were previously identified using gas chromatography electron impac
t-mass spectrometry. The same cuticular hydrocarbon peaks were identif
ied in the Asian and Brazilian populations. Samples from three populat
ions were analyzed using capillary gas chromatography chemical ionizat
ion mass spectrometry. Total carbon number and branch points were foun
d for four peaks which occurred at equivalent chain lengths >38.65. Qu
antitative analyses were performed by flame ionization gas chromatogra
phy. All populations contained the same cuticular hydrocarbons but sho
wed differences in percentage composition. Nine of the 14 populations
were separable by discriminant analysis of hydrocarbon components. Ove
rlap of the 95% CIs occurred between the two Brazilian populations and
among three North American populations. Individual specimens from thr
ee North American populations were used as a test group in the discrim
inant analysis. Correct classification of these individuals by populat
ion ranged from 80 to 100%.