USE OF AN INVIVO SYSTEM TO DETERMINE THE G418 RESISTANCE PHENOTYPE OFBLOOD-STREAM-FORM TRYPANOSOMA-BRUCEI-BRUCEI TRANSFECTANTS

Authors
MURPHY NB MUTHIANI AM PEREGRINE AS
Citation
Nb. Murphy et al., USE OF AN INVIVO SYSTEM TO DETERMINE THE G418 RESISTANCE PHENOTYPE OFBLOOD-STREAM-FORM TRYPANOSOMA-BRUCEI-BRUCEI TRANSFECTANTS, Antimicrobial agents and chemotherapy, 37(5), 1993, pp. 1167-1170
Citations number
21
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
37
Issue
5
Year of publication
1993
Pages
1167 - 1170
Database
ISI
SICI code
0066-4804(1993)37:5<1167:UOAIST>2.0.ZU;2-F
Abstract
We determined the level of susceptibility of Trypanosoma brucei brucei to the aminoglycoside G418 in vivo and demonstrated that it is possib le to select for G418-resistant transfected T. brucei brucei bloodstre am parasites in a mouse host by inoculating the drug intraperitoneally at doses between 40 and 80 mg/kg of body weight daily for 3 days. The ability to select for transfectants in vivo offers new possibilities for studies on genetic recombination in these parasites.