INHIBITION OF OXIDIZED LOW-DENSITY-LIPOPROTEIN METABOLISM IN MACROPHAGE-J774 BY HELVOLIC ACID

Authors
SHINOHARA C HASUMI K ENDO A
Citation
C. Shinohara et al., INHIBITION OF OXIDIZED LOW-DENSITY-LIPOPROTEIN METABOLISM IN MACROPHAGE-J774 BY HELVOLIC ACID, Biochimica et biophysica acta, 1167(3), 1993, pp. 303-306
Citations number
15
Categorie Soggetti
Biophysics,Biology
Journal title
Biochimica et biophysica acta → ACNP
ISSN journal
00063002
Volume
1167
Issue
3
Year of publication
1993
Pages
303 - 306
Database
ISI
SICI code
0006-3002(1993)1167:3<303:IOOLMI>2.0.ZU;2-W
Abstract
The antibiotic helvolic acid inhibited cholesteryl ester accumulation in macrophage J774 treated with oxidized low-density lipoprotein (LDL) at a concentration of 50-350 muM. The agent reduced oxidized I-125-LD L degradation and [C-14]oleate incorporation into cholesteryl ester. I n a cell-free assay, ATP-dependent acidification of endosomes and lyso somes was significantly inhibited by 35 muM helvolic acid, suggesting that this activity accounts for the inhibition of oxidized LDL metabol ism in the macrophages.