R. Drysdale et al., GENES REQUIRED FOR EMBRYONIC MUSCLE DEVELOPMENT IN DROSOPHILA-MELANOGASTER - A SURVEY OF THE X-CHROMOSOME, Roux's archives of developmental biology, 202(5), 1993, pp. 276-295
We have begun a genetic analysis to dissect the process of myogenesis
by surveying the X chromosome of Drosophila melanogaster for mutations
that affect embryonic muscle development. Using polarised light micro
scopy and antibody staining techniques we analysed embryos hemizygous
for a series of 67 deletion mutations that together cover an estimated
85% of the X chromosome, or 16.5% of the genome. Whereas the mature w
ild type embryo has a regular array of contractile muscles that insert
into the epidermis, 31 of the deletion mutants have defects in muscle
pattern, contractility or both, that cannot be attributed simply to e
pidermal defects and identify functions required for wild type muscle
development. We have defined mutant pattern phenotypes that can be des
cribed in terms of muscle absences, incomplete myoblast fusion, failur
e of attachment of the muscle to the epidermis or mispositioning of at
tachment sites. Thus muscle development can be mutationally disrupted
in characteristic and interpretable ways. The areas of overlap of the
31 deletions define 19 regions of the X chromosome that include genes
whose products are essential for various aspects of myogenesis. We con
clude that our screen can usefully identify loci coding for gene produ
cts essential in muscle development.