ANALYSIS OF THE TISSUE-SPECIFIC PROMOTER OF THE MUC1 GENE

The 5'-sequences flanking the human MUC1 gene have been analyzed for t heir ability to direct expression of a reporter gene (the chlorampheni col acetyltransferase gene (CAT)) in cell lines that normally express or do not express the MUC1 gene. A construct containing 2.9 kilobase p airs of MUC1 5'-flanking sequence sequence showed expression of CAT in breast and pancreatic cell lines but not in the non-epithelial cell l ines HT-1080, SK23, and HTB96. Deletion analysis showed that maximum e xpression was obtained in ZR-75 (breast cancer line) and HPAF (pancrea tic cancer line) with only 743 base pairs of 5'-flanking sequence. Seq uences within 1.6 kilobase pairs of the transcriptional start site sho wed enhancing activity in a vector carrying an enhancerless SV40 promo ter. Analysis of proximal 5'-sequences in a promoterless CAT vector ca rrying the SV40 enhancer showed that sequences between -60 and -150 we re crucial for tissue-specific expression. An Spl site at -99/-90 and an E box (E-MUC1) at -84/-64 in this region were shown by mutational a nalysis to play a role in the regulation of transcription. Gel shift a nalysis with oligonucleotides and nuclear extracts of ZR-75 showed pro tein binding to both of these sites. Sp1 binding activity was similar in ZR-75 and HT1080 cells, whereas binding of factors to the E-MUC1 ol igonucleotide revealed quantitative and qualitative differences betwee n epithelial and non-epithelial cells.