Plb. Bruijnzeel et al., LACK OF INCREASED NUMBERS OF LOW-DENSITY EOSINOPHILS IN THE CIRCULATION OF ASTHMATIC INDIVIDUALS, Clinical and experimental allergy, 23(4), 1993, pp. 261-269
The density distribution pattern of eosinophils over discontinuous iso
tonic Percoll gradients from the blood of normal, asymptomatic allergi
c and non-allergic asthmatic individuals was investigated. There was a
completely identical distribution pattern between the investigated gr
oups. Analysis of the expression of surface markers for complement rec
eptors CR1 and CR3 and immunoglobulin G receptor on eosinophils derive
d from the density bands 1.080, 1-085 and 1.090 g/ml supported this fi
nding since they did not reveal differences in expression between the
bands within one group but also not between the three groups. Eosinoph
ils of the various density bands were further purified and stimulated
in vitro to produce leukotriene C4 (LTC4) by the calcium ionophore A23
187 or serum treated zymosan. Equal amounts of LTC4 were synthesized b
y the eosinophils of the various density bands within one group. Howev
er, it appeared that the eosinophils of all density bands of allergic
and non-allergic asthmatics synthesized significantly more LTC4 than t
he eosinophils from normal individuals (five-to tenfold). Probably thi
s indicates in vivo priming of the eosinophils in asthmatic individual
s which is not reflected by a change in density. Control experiments,
dealing with possible artifacts due to the isolation procedure or the
patient selection, to find differences in distribution patterns over d
iscontinuous Percoll density gradients of the eosinophils of asthmatic
compared to normal individuals failed to show such a difference. Ther
efore, the density distribution pattern of eosinophils over these grad
ients does not reflect cell activation, whereas LTC4 formation clearly
does. This could mean that LTC4 formation is a more sensitive paramet
er for cell activation than density distribution or cell surface marke
r expression.