N. Fenina et al., CONCENTRATION OF TEMAROTENE (RO-15-0778) AND ITS METABOLITE RO-14-6113 IN PLASMA AND SKIN OF HAIRLESS RAT, Skin pharmacology, 6(1), 1993, pp. 61-64
The aim of this preliminary report was to measure plasma and skin conc
entrations of Ro 15-0778 and its phenolic metabolite Ro 14-6113 in hai
rless rats receiving orally 10 mg/kg of Temarotene once daily during 1
0 days. Blood (2-3 ml) and skin (200-300 mg) samples were taken at dif
ferent time points between 0.5 and 240 h after the last dose. We used
a highly sensitive HPLC method for simultaneous determination of the t
wo compounds with a quantification limit of 2 ng/ml in plasma and 10 n
g/g in total skin (epidermis and dermis). After 10 h, plasma concentra
tions of Ro 14-6113 were 5-13 times higher than for Ro 15-0778. Ro 14-
6113 concentrations in the skin were 4-10 times higher than for Ro 15-
0778 within the initial 48 h. The concentrations of both compounds in
the skin were higher than concentrations in plasma.