RESOLUTION OF DIASTEREOMERIC PHOSPHORAMIDATE BRIDGED UNNATURAL OLIGONUCLEOTIDES BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Micellar electrokinetic chromatography was used to resolve diastereome rs of oligonucleotides possessing several chiral phosphoramidate bridg es. These materials were not resolved by conventional liquid chromatog raphic techniques. Parameters that were found to affect electrophoreti c resolution included the nature and concentration of surfactant, pres ence of organic modifiers such as propan-2-ol and urea, use of deuteri um oxide-based buffers and temperature control. Practical optimization was achieved by employing Terabe's resolution principles.