Holstein cows (n = 46) were fed free choice a silage mixture balanced
weekly throughout lactation using 13 and 36% CP grains to individualiz
e CP for each cow; grains contained 15 and 20% of distillers grains wi
th solubles, respectively. Cows were blocked by parity (1 vs. > 1) and
assigned at calving to receive a commercial bypass protein-fat supple
ment at 0 (control) or 6% of weekly projected 4% FCM yield throughout
lactation. In peak lactation, supplementation raised dietary fat from
4.3 to 6.0% of DM, NE(L) from 1.64 to 1.70 Mcal/kg of DM, and undegrad
able protein from 42 to 47% of CP and contributed about 25 and 20% of
total CP in early and late lactation. Supplement reduced forage and to
tal DMI significantly, which negated the potential nutritional value o
f the supplement. Reduction in protein content of milk from supplement
ed cows was small but significant; BW and yields of milk, SCM, and 4%
FCM were not significantly affected by treatment. Supplementation incr
eased fat test in parity 1 cows and lowered it in older cows. In wk 5
to 8, 21 to 24, and 37 to 40 postpartum, cows consumed 100 to 116% of
the NRC recommendations of undegradable protein but only 65 to 94% of
degradable CP needs; NE(L) intake generally was adequate except for pr
imiparous cows in early lactation. Supplementation lowered Lys intake
in early lactation. Addition of rumen-protected fat and undegradable,
high quality protein mixture to the diet of lactating cows cannot be e
ffective if its use reduces DMI or if degradable protein intake is ina
dequate.