INTERLEUKIN-10 IMMUNOADHESIN PRODUCTION BY A REPLICATION-DEFECTIVE ADENOVIRUS

The present study examined the use of replication-defective adenovirus for in vitro production of an immunoadhesin. A recombinant adenovirus , rendered replication defective by deletion of the El gene, was const ructed to contain the murine interleukin-10 gene fused in frame with t he hinge, CH2, and CH3 domains of the murine immunoglobulin gamma 1 he avy chain constant region gene under the control of the human cytomega lovirus promoter. The resultant recombinant virus, Ad5.hCMV.mIL-10:HFc , was used to transduce several cell types. The expressed protein, mIL -10:HFc, is secreted as a disulfide-bonded homodimer. In vitro, a muri ne pro-B-cell line expressing transfected recombinant murine interleuk in-10 receptor proliferated in response to purified mIL-10:HFc. The re sults obtained demonstrate the relative ease of production of an immun oadhesin using replication-defective adenovirus.