A MURINE MODEL OF PULMONARY DAMAGE-INDUCED BY LIPOPOLYSACCHARIDE VIA INTRANASAL INSTILLATION

This study examines the intranasal instillation of lipopolysaccharide (LPS) into BALB/c mice causing acute pulmonary damage, due to neutroph il infiltration and sepsis. A dose response with LPS showed that an in tranasal instillation of 167 mu g/ml(10 mu g/mouse) caused acute lung injury within 2-4 h and reached maximal damage at 24-48 h. We found th e method of LPS administration for induction of acute pulmonary damage to be crucial, After 24 h post-LPS injection, a comparison showed a s ubstantial increase in pulmonary damage with intranasal instillation o f LPS. As for intravenous injection, it showed a baseline effect. This study indicates that LPS administered intranasally causes acute pulmo nary damage, whereas with intravenous and intraperitoneal endotoxin ad ministration a tissue-specific or similar degree of pulmonary injury m ay not develop.