WESTERN-BLOT-ANALYSIS OF SERA FROM LYME BORRELIOSIS PATIENTS ACCORDING TO THE GENOMIC SPECIES OF THE BORRELIA STRAINS USED AS ANTIGENS

Sera of 52 Lyme borreliosis patients classified according to their cli nical features were analysed by Western blot using as antigens Borreli a strains belonging to three recently described genomic species. The a ntibody response was demonstrated to be homologous within each genospe cies. Serum reactivity was studied for each of the type strains Borrel ia burgdorferi sensu stricto (strain B31T), Borrelia garinii (strain 2 0047T) and group VS461. Seven of 15 sera (46.6 %) of patients with men ingoradiculitis showed preferential reactivity with Borrelia garinii ( strain 20047T), all of 8 sera (100 %) of patients with acrodermatitis chronica atrophicans showed preferential reactivity with group VS461 ( strain VS461) and 8 of 16 sera (50 %) of patients with arthritis showe d preferential reactivity with Borrelia burgdorferi sensu stricto (str ain B31T). The presence of a strong response to OspA and OspB proteins of Borrelia burgdorferi sensu stricto was found only in this last gro up of patients. These results suggest that there are clinical implicat ions of the recently described modifications in the taxonomy of Borrel ia burgdorferi.