P. Minning et al., THE DEVELOPMENT OF AN ENZYME-IMMUNOASSAY (EIA) FOR DETECTION OF AVIANAND MOUSE ANTIBODIES AGAINST THE NEWCASTLE-DISEASE VIRUS, Berliner und Munchener Tierarztliche Wochenschrift, 106(5), 1993, pp. 149-153
The development of an enzyme-immuno-assay (EIA) for detection of avian
and mouse antibodies against the Newcastle-Disease Virus is shown. Th
e antigen preparation was the centre of the experiments. The basic ass
ay programme and selected test steps were optimized. The established a
ssay is part of the EIA-control system for a SPF (specific-pathogen-fr
ee)-flock and also the essential screening method for the production o
f anti-NDV-monoclonal antibodies.