CHAPERONINS DEPENDENT INCREASE OF CU,ZN SUPEROXIDE-DISMUTASE PRODUCTION IN ESCHERICHIA-COLI

Authors
BATTISTONI A CARRI MT STEINKUHLER C ROTILIO G
Citation
A. Battistoni et al., CHAPERONINS DEPENDENT INCREASE OF CU,ZN SUPEROXIDE-DISMUTASE PRODUCTION IN ESCHERICHIA-COLI, FEBS letters, 322(1), 1993, pp. 6-9
Citations number
23
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
322
Issue
1
Year of publication
1993
Pages
6 - 9
Database
ISI
SICI code
0014-5793(1993)322:1<6:CDIOCS>2.0.ZU;2-F
Abstract
Over-expression of the chaperonins GroEL and GroES significantly suppr essed the temperature-dependent pattern of expression of Cu,Zn superox ide dismutases in Escherichia coli and increased the yield of active e nzyme. The results obtained indicate that chaperonins prevent degradat ion of metal-deficient enzyme molecules. GroEL was shown to form a com plex with unfolded Cu,Zn superoxide dismutase in vitro, confirming tha t GroEL can interact with beta-stranded proteins.