Yk. He et al., INVITRO CLEAVAGE OF HPV16 E6 AND E7 RNA FRAGMENTS BY SYNTHETIC RIBOZYMES AND TRANSCRIBED RIBOZYMES FROM RNA-TRIMMING PLASMIDS, FEBS letters, 322(1), 1993, pp. 21-24
A RNA-trimming plasmid pRG523 is constructed, in which three Rz genes,
GR5(5'-cis-Rz gene), HR2G(trans-Rz gene) and GR3(3'-cis-Rz gene), are
arranged in the order from 5' to 3' downstream from the T7 promoter.
In vitro transcription of this plasmid shows that the trans-Rz can be
trimmed to definite lengths by the cis-Rz on both sides of the trans-R
z. In vitro cleavage of HPV16 E6 and E7 RNA fragments of different len
gths by synthetic Rz and that of E7 RNA with a length of 171 nt by syn
thetic Rz and transcribed Rzs with different lengths of flanking seque
nces is studied. The results show that the non-base-pairing flanking s
equences on both Rz and target RNA can affect the cleavage reaction.