INVITRO CLEAVAGE OF HPV16 E6 AND E7 RNA FRAGMENTS BY SYNTHETIC RIBOZYMES AND TRANSCRIBED RIBOZYMES FROM RNA-TRIMMING PLASMIDS

Authors
Citation
Yk. He et al., INVITRO CLEAVAGE OF HPV16 E6 AND E7 RNA FRAGMENTS BY SYNTHETIC RIBOZYMES AND TRANSCRIBED RIBOZYMES FROM RNA-TRIMMING PLASMIDS, FEBS letters, 322(1), 1993, pp. 21-24
Citations number
14
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
322
Issue
1
Year of publication
1993
Pages
21 - 24
Database
ISI
SICI code
0014-5793(1993)322:1<21:ICOHEA>2.0.ZU;2-9
Abstract
A RNA-trimming plasmid pRG523 is constructed, in which three Rz genes, GR5(5'-cis-Rz gene), HR2G(trans-Rz gene) and GR3(3'-cis-Rz gene), are arranged in the order from 5' to 3' downstream from the T7 promoter. In vitro transcription of this plasmid shows that the trans-Rz can be trimmed to definite lengths by the cis-Rz on both sides of the trans-R z. In vitro cleavage of HPV16 E6 and E7 RNA fragments of different len gths by synthetic Rz and that of E7 RNA with a length of 171 nt by syn thetic Rz and transcribed Rzs with different lengths of flanking seque nces is studied. The results show that the non-base-pairing flanking s equences on both Rz and target RNA can affect the cleavage reaction.